32 DNA molecules would there be after four rounds of PCR if the initial reaction mixture contained two molecules.
- A single DNA molecule can be amplified by the PCR method up to 2n times, where n is the number of cycles. A given DNA template can therefore be amplified to 16 duplicate strands using PCR over 4 cycles.
- PCR doubles the amount of target DNA molecules per cycle, assuming there are enough primers, dNTPs, and polymerase molecules.
- Five cycles equal five times the target DNA is doubled. 32 is equal to 2 to the fifth power, 2x2x2x2x2.
- The following equation can be used to determine how many copies of double-stranded DNA are present overall:
DNA copies are calculated as follows = (DNA quantity (ng) x 6.0221023 / (DNA length x 1109 ng/ml x 650 Daltons)
- The amount of template required for each reaction is determined by calculating the number of DNA copies.
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