if mrnas could be ligated and replicated within plasmids, what enzyme commonly used in recombinant dna technology would no longer be needed?

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08-12-2022
Biology

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if mrnas could be ligated and replicated within plasmids, what enzyme commonly used in recombinant dna technology would no longer be needed?

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aashvi1 aashvi1 · Answer 1 · 2022-12-19T12:23:51+01:00

An enzyme called reverse transcriptase is employed to make complementary DNA (cDNA) from RNA. To create a single, intact DNA molecule, the enzyme DNA ligase binds the fragments with complementary ends.

What is recombinant DNA?

As a result, a recombinant plasmid containing the target gene is created. The process of fusing two or more DNA molecules to produce a hybrid is known as recombinant DNA. Restrictions endonucleases and ligases are two classes of enzymes that enable the technique. Restrictions enzymes, polymerases, and ligases are among the enzymes that aid in cutting, synthesis, and binding. The position at which the desired gene is inserted into the vector genome is greatly influenced by the restriction enzymes utilized in recombinant DNA technology.

To learn more about Restrictions from given link

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